Overview of Core
Provides instrumentation, as well as technical and professional assistance, for performing laser-based analytical flow cytometry and cell sorting.
- Polychromatic subset enumeration, quantification of antigen expression levels, measurement of cell cycle distribution and DNA content, measurement of intracellular antigens (alone or combined with DNA content analysis and/or cell surface immunofluorescence), calcium flux evaluation (alone or combined with cell surface immunofluorescence), apoptosis measurements (alone or combined with DNA content analysis or cell surface immunofluorescence), measurement of DNA-RNA content and assessment of fluorescent protein expression. The core facility provides high-speed aseptic sorting, sorting of potentially biohazardous specimens, and single cell cloning. Sorting options include the use of various sort nozzle sizes and instrument pressures.
- Consistent and extensive instrumentation quality control.
- Consultation with the manager, as well as continuous availability of expert advice on flow cytometry during data collection and analysis, through personnel in the laboratory.
- Education through formal courses is provided as needed to users, so they may learn basic flow cytometry principles and learn to run their own specimens on the analytic flow cytometers under supervision of the shared resource staff. Classes on nucleic acid flow cytometry are also offered.
There is a tiered fee structure. Costs for JCCC members are listed below; for non-members, please refer to our website
|Calcium flux measurements
|Acquisition and analysis by FCSR staff (sample stained by researcher)
|Acquisition and analysis by researcher, with supervision by FCSR staff
|Sample stained with antibody provided by FCSR laboratory and
data acquired and analyzed by FCSR staff (2 tube minimum)
|Reagent purchase by researcher from FCSR
||$4 - $8/aliquot
|Data storage devices
||$35 - $60
|Flow cytometry class
|Nucleic acid flow cytometry instructional session
|Off-line computer analysis by researcher
|Use of the RoboSep cell separator
||$30/ 45 mins
Faculty investigators, research fellows, or others who wish to start using the Flow Cytometry Shared Resource are encouraged to contact the core manager for discussion of their experiments, help with study design, and assistance with the procedures and product descriptions of reagents they will need to order. Pilot studies are performed, if necessary, and the study design is perfected. The core manager remains actively involved in the more complex projects and oversees the technical quality of all work produced by the laboratory. With the exception of consultation, developmental work or pilot experiments, the principal investigators are recharged for all time and/or work performed. Details are available on the core website.
12-236 Factor Building - office
12-953 Factor Building - main laboratory